CD90.2 MicroBeads Mouse

University of Montana
DBS
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Miltenyi Biotec

Product Name:

CD90.2 MicroBeads mouse

Catalog Number:

130-049-101

We perform both depletion and selection of multiple cell types from cell cultures as well as whole tissues including spleen, lymph nodes, thymus, lung, and bone marrow. Cell samples are then used for RT-qPCR, Flow Cytometric analysis, and re-culturing for functional studies.

Experimental Design and Results Summary

Application

CD90.2+ cell purification

Starting Material

Mouse single cell suspension

Protocol Overview

After suspending cells in compatible buffer, add CD90.2+ microbeads to cell suspension. Incubate cells and perform wash prior to adding cells to appropriate magnetic column.

Tips

Filter cells before adding to column to reduce clogs

Results Summary

Timely and straightforward way to enrich CD90.2+ cells for downstream applications.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Easy to use and few steps.

The Bad

Clogged columns if preforming manual purification

The Bottom Line

Nice and easy way to purify CD90.2+ cells on the bench top in relatively short amount of time.

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