CD11c MicroBreads UltraPure Mouse

University of Montana
DBS
Graduate Student

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Quality of Results

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Company:

Miltenyi Biotec

Product Name:

CD11c MicroBreads UltraPure mouse

Catalog Number:

130-108-338

We use the perform both depletion and selection of multiple cell types from cell cultures as well as whole tissues including spleen, lymph nodes, thymus, lung, and bone marrow. Cell samples are then used for RT-qPCR, Flow Cytometric analysis, and re-culturing for functional studies.

Experimental Design and Results Summary

Application

Selection of CD11c+ cells

Starting Material

Mouse single cells suspension

Protocol Overview

Prepare cells in compatible buffer at desired concentration, filter and load cells to column held in magnetic separator, wash three times with buffer, and collection positively selected cells.

Tips

Filter cells immediately before adding to column to reduce occurrence of slow flow

Results Summary

We consistently achieve >98% purity of CD11c+ cells using this kit. Cells appear functional and minimally affected for use in downstream assays.

DOI or PMID #

N/A

Additional Notes

N/A

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Summary

The Good

Quick, easy, and cost efficient

The Bad

None

The Bottom Line

With minimal steps for preparing cells, this kit is a quick and easy way to increase purity of CD11c+ cells.

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