Working Zinc Transporter 1 Antibody

August 03, 2017

BlueskyReddit
Biophysics
National Institute Of Nutrition, Hyderabad
Ph.D Scholar

Overall

Quality of Results

Ease-of-Optimization

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Company:

ALPHA DIAGNOSTICS

Product Name:

Zinc Transporter 1

Catalog Number:

ZIP11-A

Browse Similar Products: ZIP1 Western Blot Antibodies

ZIP11 is a membrane transporter. It transports zinc. I treated the cells to see whether they are ZIP1 expressing or not.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Caco2 cell lysate

Primary Incubation

1:1000 dilution, overnight at 4 degrees

Blocking Agent

BSA dissolved in 1x TBST

Secondary Incubation

1:5000 dilution in 1x TBST, 1hr, room temperature

Tertiary Incubation

None

Detection

ECL substrate used developed by G-BOX chemiluminiscent

Results Summary

I got results from this antibody, but nonspecific bands came

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Clear band will come

The Bad

Multiple bands

The Bottom Line

Working antibody but nonspecific bands came

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