R&D Systems
Anti-mouse arg1 pe
ic5868p
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The purpose was to differentiate between macrophage and dendritic cell populations based on arg1 expression. This product was selected because of the fluorochrome availability.
Flow Cytometry
Mouse bone marrow derived cells
30 minutes on ice in permeabilization buffer
FC block 1:100
None
The results show that bone marrow derived dendritic cells and macrophage populations all express Arg1 to a high extent. Arg1 does not differentiate between these populations, unlike what the literature claims.
N/A
Stains brightly
Does not differentiate between cell populations
Try a different antibody to differentiate between macrophage subsets