Working Secondary Antibody

August 02, 2017

Biophysics
National Institute Of Nutrition, Hyderabad
Ph.D Scholar

Overall

Quality of Results

Ease-of-Optimization

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Company:

SIGMA

Product Name:

Anti-Rabbit IgG (whole molecule)-Peroxidase

Catalog Number:

A0545

Browse Similar Products: IgG Western Blot Antibodies

I used this secondary antibody for western blotting. I have many primary antibodies for anti rabbit.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Caco2 cell lysate

Primary Incubation

1:1000 dilution, overnight, 4 degrees

Blocking Agent

BSA dissolved in 1x TBST

Secondary Incubation

1:5000 dilution in 1x TBST, 1hr, room temperature

Tertiary Incubation

None

Detection

ECL substrate used developed by G-BOX chemiluminiscent

Results Summary

This secondary antibody gives clear bands with more dilution. With lower dilution it gave high background images. It was good working antibody.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Clear band will show up

The Bad

Sometimes background will show up

The Bottom Line

With a greater dilution it is working well and less dilution will give more background.

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