Biolegend
Brilliant Violet 711™ anti-mouse CD4 Antibody
100557
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This review displays the use of BV711 CD4 antibody to detect tumor infiltrating lymphocytes in B16F10 mouse melanoma tumors. Tumors were dissociated, blocked, stained and run on BD FACS Aria.
Flow Cytometry
B16F10 mouse melanoma tumor
1:100 dilution (1 million cells/100 μl)
Mouse BD Fc Block (1 μg/million cells in 100 μl)
NA
BD FACS ARIA
Lymphocytes were first gated by size on a FSC vs SSC plot. Live cells were then gated (DAPI negative) and analyzed for CD4. We achieved excellent separation from CD4 negative cells. Results are shown as dot plot and histogram.
Easy to use
This fluorochrome is a bit pricey.
Use this antibody to detect CD4+ tumor infiltrating lymphocytes.