Thermo Scientific
DreamTaq Green PCR Master Mix (2X)
K1081
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We use this PCR master mix to genotype our mouse lines. It is quick and easy to set up and generates reliable results. The product can be loaded directly into agarose gels without the need to mix with loading buffer.
PCR
Predesigned primers and template DNA
Mix master mix with primers and template DNA and run a standard PCR cycle. Each PCR reaction will need to be optimized.
Make sure to run a positive and negative control
Once a protocol is optimized, you should be able to produce clean DNA product. This master mix is best used for applications where DNA is not used for downstream cloning. Perfect for quick genotyping applications
N/A
Quick and easy
Need to be optimized
Great for routine PCR amplification