Applied Biosystems by Thermo Fisher Scientific
TaqMan Universal PCR Master Mix II, no UNG
4440040
This qPCR master mix is used in standard qPCR assays where probe base primers are used. Levels of mRNA in cells and tissues can be quantified using this qPCR master mix.
qPCR
cDNA and targer primer
Add fluorescently labeled target specific probes, cDNA and master mix to qPCR plate and run using a standard qPCR protocol on a qPCR machine
Try be as accurate as possible when pipetting
Amplification curves, disassociation curves and Ct values are used to quantify relative gene levels
N/A
Easy to use!
qPCR can be tricky and pippetting needs to be very accurate
A great mastermix for probe based qPCR assays