Excellent Antibody

Cell Biology
Sloan-Kettering Institute
PHD Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Sigma

Product Name:

Anti-Acetylated tubulin antibody

Catalog Number:

T7451 SIGMA

Acetylated alpha tubular antibody stains microtubules brightly. Works with PFA fixations or methanol. The IgG2b isotope is convenient.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

RPE1 cells that have been treated with nocodazole for 1 hour

Primary Incubation

1:1000 dilution in PBS with 3% BSA and 0.1% triton

Blocking Agent

PBS with 3% BSA and 0.1% triton

Secondary Incubation

Molecular probes anti mouse IgG2b 594 secondary antibody in PBS with 3% BSA and 0.1% triton

Tertiary Incubation

DAPI

Detection

Immunofluorescence

Results Summary

Stable microtubules that resist nocodazole treatment are stained brightly and clearly

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

It is an IGG2b antibody allowing containing with multiple other IgGs

The Bad

Nothing Bad at all

The Bottom Line

It is truly a great antibody to stain acetylated microtubules, Excellent for cilia.

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