Isotype Control For APC Labeled Antibodies

August 09, 2017

Pharmaceutical Technology
CIBER BBN
Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

Biolegend

Product Name:

APC Rat IgG2a, κ Isotype Ctrl Antibody

Catalog Number:

400511

We use this antibody as an isotype control for IgG2a, k antibodies labeled with APC dye. It helps to discern unspecific signal from specific labeling.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Single cell suspension from mouse spleen

Primary Incubation

1:100

Blocking Agent

We block with CD16/32

Secondary Incubation

None

Tertiary Incubation

None

Detection

We detect in the channel for APC in the flow cytometer

Results Summary

It is very helpful to discern nonspecific signal provided by APC Rat IgG2a, kappa antibodies from specific signal.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Good price

The Bad

None

The Bottom Line

I highly recommend it for isotype control

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