Working MTP1 Antibody

Biophysics
National Institute Of Nutrition, Hyderabad
Ph.D Scholar

Overall

Quality of Results

Ease-of-Optimization

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Company:

Alpha Diagnostics International

Product Name:

MTP1 Antibody

Catalog Number:

MTP11-S

Browse Similar Products: MTP1 Western Blot Antibodies

Metal Transporter Protein (MTP1) is also described as an iron-regulated transporter (IREG1), Ferroportin1, and SLC11A3 iron transporter or solute carrier family 11 (proton-coupled divalent metal ion transporter), Member 3, formerly SLC11A3). MTP1 contains IRE in the 5’-UTR. It is expressed in tissues involved in body iron homeostasis. In this, I have studied the regulation of mtp1 and activated pathway with different stimuli.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Caco2 cell lysate

Primary Incubation

1:1000 dilution,over night,4 degrees

Blocking Agent

BSA dissolved in 1x TBST

Secondary Incubation

1:4000 dilution,1hr,room temperature

Tertiary Incubation

None

Detection

ECL substrate used developed by G-BOX chemiluminiscent

Results Summary

I got good results but it will give multiple bands sometimes.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Clear band will come

The Bad

Multiple bands sometimes

The Bottom Line

It was good working antibody,but multiple bands will come

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