Cell Signaling Technology
Akt (pan) (C67E7) Rabbit mAb
4691
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Pretreatment of zinc induces increased iron absorption compared to iron alone, iron and iron, and zinc together. We are investigating the mechanism of zinc induced iron pathways. We are checking the pi3k/akt/mTOR pathway.
Western Blot
Caco2 cells treated with zinc lysed in RIPA buffer
1:1000 dilution,overnight at 4 degrees
5% Bovine serum albumin dissolved in 1%TBST
1:4000 dilution,1hr room temparature
None
ECL substrate used developed by G-BOX chemiluminiscent
I got good results.
N/A
Single band will come up
Nothing
This antibody works every well.