Santa Cruz Biotechnology, Inc.
NRAMP2
SC-166884
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NRAMP2 also called DMT1 is a membrane iron transporter. In intestinal epithelial cells, it absorb the Fe from dietary sources.
Western Blot
Caco2 cells treatment with zinc
1:5000 dilution, overnight at 4 degrees
BSA dissolved in 1x TBST
1:200, 1 hr, room temperature
None
ECL substrate used developed by G-BOX chemiluminiscent
I am getting good results with this protocol.
N/A
Very good
High intensity
This is very good product, but the brightness of the bands are more than expected. The bands are saturated if we take more concentration of protein. If we take less protein concentration, we will get good results.