Great Antibody For CD4+ T Cell Detection

July 27, 2017

BlueskyReddit
Cell Biology
Albert Einstein College of Medicine
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Invitrogen Antibodies

Product Name:

CD4 Monoclonal Antibody (S3.5), TRI-COLOR

Catalog Number:

MHCD0406

The general aim of the research is to study hematopoiesis and acute myeloid leukemia. This antibody was used to detect and isolate CD4+ cells from the human blood samples.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human peripheral blood

Primary Incubation

1:100

Blocking Agent

Fc block is added to cells prior to staining with antibody

Secondary Incubation

None

Tertiary Incubation

None

Detection

Flow cytometry on a BD LSRII-yellow machine

Results Summary

Strong signal and good separation from other human blood cell populations

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Strong signal

The Bad

None

The Bottom Line

Recommended for flow cytometry on human blood cells

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