eBioscience™ from Invitrogen Antibodies
CD45.2 Monoclonal Antibody (104), APC
17-0454-82
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The general aim of the research is to study hematopoiesis and acute myeloid leukemia. This antibody was used to identify transplanted CD45.2 cells in CD45.1 recipient mice.
Flow Cytometry
Murine bone marrow
1:100
Fc block is added to cells prior to staining with antibody
None
Flow cytometry on a BD LSRII-yellow machine
Good separation from CD45.1 and ability to detect CD45.2 recipient cells by flow cytometry
N/A
Strong, detectable signal
The antibody gives a strong signal and allows for easy identification of the CD45.2+ population