AFP Levels In Human Plasma

July 14, 2017

Neurobiology
St. Jude Children's Research Hospital

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Company:

R&D Systems

Product Name:

Human/Rat alpha-Fetoprotein/AFP antibody

Catalog Number:

AF1369

Alpha-Fetoprotein levels in normal and preeclamptic serum samples were analyzed by western blot analysis.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Human Plasma

Primary Incubation

Anti-AFP antibody (Polyclonal Chicken IgY)

Blocking Agent

5% skim milk

Secondary Incubation

Goat anti-Chicken IgY-unconjugated

Tertiary Incubation

Donkey anti-goat IgG-HRP

Detection

Chemiluminescence

Results Summary

The AFP levels were relatively high in preeclamptic plasma samples compared to normal condition.

DOI or PMID #

N/A

Additional Notes

N/A

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Summary

The Good

Very good antibody with a little background

The Bad

Hard to find anti-chicken HRP conjugated secondary antibodies. So it needs a tertiary antibody for the detection!

The Bottom Line

Very good antibody for western blot applications.

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