PD-1 Expression In T Cells

July 21, 2017

Immunology
Northwestern University
Grad Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

eBioscience™ from Invitrogen Antibodies

Product Name:

CD279 (PD-1) Monoclonal Antibody (J43), PerCP-eFluor 710

Catalog Number:

46-9985-80

The aim of the project was to measure PD-1 expression in T cells of SRBC immunized mice.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse spleen

Primary Incubation

1: 100, 4 degrees, 30 min

Blocking Agent

Fc block

Secondary Incubation

None

Tertiary Incubation

None

Detection

None

Results Summary

Clear separation of PD-1+ population

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Easy to use, no troubleshooting required, comes ready to use

The Bad

None

The Bottom Line

Great antibody for measuring PD-1 expression.

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