Mouse On Mouse Immunodetection Kit Worked Great For Paraffin Embedded Sections

July 12, 2017

SUNY Downstate Medical Center
Department of Cell Biology
PhD Candidate (Graduate Student)

Overall

Quality of Results

Ease-of-Optimization

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Company:

Vector Laboratories

Product Name:

M.O.M. Immunodetection kit

Catalog Number:

PK-2200

We study a KO mouse model of cobalamin deficiency and have used M.O.M. kit to perform immunohistochemistry using a mouse monoclonal primary Ab. It worked great with almost no background.

Experimental Design and Results Summary

Application

Immunohistochemistry

Starting Material

Paraffin embedded mouse brain sections

Protocol Overview

Prior to blocking, we incubated the sections with 3% hydrogen peroxide. The rest of the protocol was followed exactly the way it is provided by the manufacturer. We incubated the sections with primary antibody for 30-60 minutes. A control was set with no primary Ab added to the reagent that was used for primary Ab dilution.

Tips

Make a fresh dilution of all the reagent whenever required.

Results Summary

We obtained immunostaining as desired with almost no background in mouse hippocampus. The results showed clear differences between WT and KO mouse staining indicating lower expression of the protein of interest in the KO mouse.

DOI or PMID #

28545069

Additional Notes

N/A

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Summary

The Good

Easy to follow the protocol and gives no background

The Bad

Needs too many reagents with multiple incubation times

The Bottom Line

This product makes it possible to use mouse monoclonal Abs on mouse tissues. It also gives high quality results.

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