LifeSpan BioSciences
anti-Synaptophysin Antibody
LS-C203763
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Browse Similar Products: SYP Immunohistochemistry Antibodies
We are studying how cobalamin deficiency alters learning and memory using a KO mouse model. The KO mouse showed learning disabilities and changes in early expression of long-term potentiation (LTP). TO determine whether the changes in LTP are due to alterations in the pre-synaptic or post-synaptic site, we studied the expression of synaptophysin in the hippocampus of the mouse by IHC-P.
Immunohistochemistry
Paraffin embedded mouse brain sections
1:200 dilution, 8 hrs at 4 degree C
Normal horse serum
HRP conjugated anti-rabbit IgG
NA
DAB staining
Staining was observed in the stratum radiatum region of hippocampuse in both econtrol and the KO mouse.
28545069
Antigen retrieval was performed prior to immunostaining
Easy to use
Needs optimization as lower dilution gives a huge background
Worked well on mouse brain sections and using HEP-DAB staining method. However, it might give superior images with immunofluorescence.