MilliporeSigma
anti-GluR2 Ab
AB1768-I
Browse Similar Products: GluR2 Immunofluorescence Antibodies
We are studying the neurologic deficits that occur due to cobalamin deficiency that affect learning and memory. We have a CD320 gene KO mouse model that are obtuse in learning a spatial memory task. Further, studying long-term potentiation (LTP), we found changes in early expression but not later expression of LTP. We hypothesized that the changes we observe in LTP results are dependent on the levels of GluR1 and GluR2 contiaining AMPA receptors. Hence, we studied GluR2 expression levels on mouse hippocampus by immunohistochemistry.
Immunofluorescence
Paraffin embedded mouse brain sections
1:200, 22 hrs at 4 degree C
Normal goat serum
1:200, 16 hrs at 4 degree C
NA
Fluorescence
We observed expression of GluR2 (by fluorescence) in the hippocampus of control and the KO mouse. The levels of flurescence measured (representing GluR2 expression) were similar in control and the KO mouse.
28545069
Antigen retrieval was performed prior to immunostaining
Easy to use; got results in first trial
Needed at least overnight incubation
The GluR2 Ab worked wonderfully on mouse brain sections.