Fluorescent Secondary Antibody For IF Staining

July 10, 2017

Cell Biology and Molecular Genetics
University of Maryland, College Park
Post-doctoral researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

Invitrogen Antibodies by Thermo Fisher Scientific

Product Name:

Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 568

Catalog Number:

A-11036

Our lab performs immunofluorescent staining to quantify cell types from tissues taken from in vivo models. The need for species-specific secondaries in various colors is critical.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Mouse brain section

Primary Incubation

Anti-Olig2 (Rabbit primary)

Blocking Agent

5% milk 5% goat serum

Secondary Incubation

Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 568

Tertiary Incubation

DAPI

Detection

Fluorescent microscope

Results Summary

Great staining of the intended target with little to no background.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Little to no background, very little antibody needed

The Bad

None

The Bottom Line

Great antibody for IF

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