Plasmid DNA Extraction

Durban University of Technology
IWWT
Post doctoral Fellow

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

Qiagen

Product Name:

Qiagen Plasmid mini kit

Catalog Number:

12125

I am working on the development of molecular techniques for the detection of bacteria in the environmental samples. I used this kit for extraction of plasmid DNA from bacterial cultures.

Experimental Design and Results Summary

Application

Extraction of plasmid DNA from bacterial cultures

Starting Material

Bacterial culture

Protocol Overview

Protocol is quick and easy.

Tips

Slight modification at elution step. To get a good quality DNA for sequencing, spin dry the column for over 10 min at full speed before elution, to remove ethanol.

Results Summary

The results obtained were of good quality purity of plasmid was suitable for further cloning purposes.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Easy and quick protocol

The Bad

None

The Bottom Line

Easy and quick protocol with good quality results.

Join the discussion