Efficiency of Maxima Hot Start PCR Master Mix

Durban University of Technology
IWWT
Post doctoral Fellow

Overall

Quality of Results

Ease-of-Optimization

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Company:

Thermo Scientific

Product Name:

Maxima Hot Start PCR Master Mix

Catalog Number:

K1052

I am working on the development of molecular techniques for the detection of bacteria in the environmental samples. The Maxima Hot Start PCR Master Mix was used in amplifying DNA from samples collected from wastewater treatment plants.

Experimental Design and Results Summary

Application

Polymerase Chain Reaction

Starting Material

Genomic DNA

Protocol Overview

Good and easy protocol

Tips

Be very careful in pipetting to avoid any carryover contamination

Results Summary

The results obtained were good and reproducible.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

The hot start mechanism prevents the extension of non-specifically annealed primers and prevents the formation of primer dimers.

The Bad

None

The Bottom Line

The Maxima Hot Start PCR Master Mix provides high PCR specificity and sensitivity.

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