Mouse IL-10 ELISA kit

July 03, 2017

University of Connecticut Health Center
Department of Immunology
Postdoctoral Fellow

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

Biolegend

Product Name:

Mouse IL-10 ELISA MAX™ Standard

Catalog Number:

431412

Used this kit to assess IL-10 levels in the serum and in cell culture supernatants. The assay was pretty easy to perform and the assay had a nice range of detection.

Experimental Design and Results Summary

Application

ELISA

Starting Material

Mouse serum and Supernatants from bone marrow derived macrophages

Protocol Overview

Pre-working day (24h before working).Dilute your Capture antibody in the Coating buffer and add 100 microliter/well to the 96 well/plate.Next day, Wash plate and block it for 1 hour with Assay diluent. After the blocking time wash and add your samples and standards and incubate for 2h in RT (room temperature). After 2h of incubation wash again and add the diluted Detection antibody (incubate for 1h). Then wash again and add the enzyme (diluted in Assay diluent) and incubate for 30 min., then wash again and add the TMB Substrate solution. In the end use the Stop solution. Read the plate in a plate reader

Tips

Best when run your samples fresh.

Results Summary

Was able to detect levels of cytokines reliably in serum and cell culture supernatants.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Very reliable and easy to use.

The Bad

Not that great when it comes to low concentration of cytokines in the samples.

The Bottom Line

Is highly easy to use, and very reliable. Also was cheap to perform when you are running a large number of samples.

Join the discussion