Convenient Tool To Gauge RNA

The Gladstone Institutes
GIVI
Research Associate

Overall

Performance

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

Thermo Fisher Scientific

Product Name:

Nanodrop 2000c

Catalog Number:

ND-2000C

Our lab uses LCMV (Lymphocytic Choriomeningitis Virus) as a model pathogen to study T-cell mediated immunity in the genital mucosa. Various strains of transgenic mice are inoculated and the hosts viral load is measured at various time points in different lymphatic organs. Thus, it is essential to measure consistent RNA concentrations for prior to qPCR reaction to measure load. After months of using this spectrophotometer, I'd highly recommend it!

Experimental Design and Results Summary

Application

Measuring concentrations of nucleic acids

Starting Material

RNA/DNA/cDNA

Protocol Overview

RNA/DNA can be diluted in small volumes. Start machine and run program.

Tips

Ensure the nanodrop is cleaned after each sample!

Results Summary

Results are reliable and and easily copied toward a spreadsheet format. 1 uL is all it takes to obtain results, if you have small volumes, this is excellent!

Features Summary

Can also measure proteins and cell cultures!

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Accurate and efficient

The Bad

An uncleaned stage yields unreliable results

The Bottom Line

This instrument is extremely quick and one can speedily go through multiple samples.

Join the discussion