JAM-A Human Gingival Tissue Immunofluorescence Staining

UIC
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Quality of Results

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Company:

Santa Cruz Biotechnology, Inc.

Product Name:

JAM-A Antibody (J10.4)

Catalog Number:

sc-53623

The aim is to stain JAM-A expressed by human gingival epithelial cells using immunofluorescence.  The antibody works great.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Human gingival tissue

Primary Incubation

4C overnight

Blocking Agent

10% goat serum

Secondary Incubation

Goat anti mouse Alexa Fluor 488

Tertiary Incubation

NA

Detection

Fluorescence

Results Summary

The staining is specific with little background. We tried both frozen section fixed with acetone and paraffin embedded section without antigen retrieval. Dilution 1/100-400.

DOI or PMID #

NA

Additional Notes

NA

Related Categories

Image Gallery

Summary

The Good

Specific

The Bad

No

The Bottom Line

It works great.

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