Dog HLA DRA Immunophenotyping

June 21, 2017

BlueskyReddit
Veterinary Diagnostic Laboratory
Kansas State University
RA

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Company:

Novus Biologicals

Product Name:

HLA DRA Antibody (169-1B5.2) [Allophycocyanin]

Catalog Number:

NBP2-47722APC

The aim of this experiment was phenotyping dog cells and then helping in the diagnosis of lymphoma or leukemia in dogs.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Whole dog blood in EDTA

Primary Incubation

30 mins in dark at 4 degrees

Blocking Agent

N/A

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

BD Fortessa

Results Summary

This antibody helped in the detection of HLADR antigen also the MHCII antigen that is presented B and activated T cells. Positive staining was seen.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Works well in dog species.

The Bad

None

The Bottom Line

Will buy again

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