Invitrogen Antibodies
CEP170 Monoclonal Antibody (72-413-1)
41-3200
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Browse Similar Products: CEP170 Immunofluorescence Antibodies
Mature centrioles have assessor structures (called distal and subdistal appendages) attached near their distal ends. My work attempts to elucidate the function of the subdistal appendages of the centriole. Cep170 had been previously reported to localize to both centriole proximal ends and subdistal appendages. We confirmed its localization. Kif2a, another centriolar protein had similar localization. We also needed this reliable antibody to confirm depletion of the proteins with RNAi and CRISPR. The antibodies also allowed us to determine which conditions lead to the mislocalization of these proteins (Cep128, Centriolin, ODF2, Kif2a, cep170, ninein ……).
Immunofluorescence
RPE1 cells (human derived). Also works with mouse derived cell lines. Works in 4% PFA fixation of for methanol fixation
1:1000 dilution in PBS with 3% BSA and 0.1% triton
PBS with 3% BSA and 0.1% triton
Molecular probes anti mouse IgG1 594 secondary antibody in PBS with 3% BSA and 0.1% triton
DAPI
Fluorescent microscopy
Cep170 antibody works well for immune fluorescence. Brighter with PFA fixation than with methanol. Brighter signal seen when staining human derived cell lines than those derived from mouse tissue.
27818179
N/A
It stains the centriole subdistal appendages and centriole proximal ends
Quality of stains decline if stored at 4C for over 1 year.
This is a reliable Cep170 antibody.