Excellent CD104 Antibody

Urology
UT Southwestern Medical Center
Research Associate

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

a-hu CD104 PE

Catalog Number:

327807

Flow cytometry was used to probe epithelial dynamics and the immune infiltrates in human prostate tissues. Epithelia was investigated with CD45-CD326+ cells. Leukocytes were looked at using CD326-CD45+. Sub-populations (such as epithelial stem cells or individual leukocyte populations like T-Cells) were gated with appropriate markers. This antibody was used to identify basal epithelia.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Primary Human Prostate Cells

Primary Incubation

1:20 25 mins

Blocking Agent

FcX

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

FACS

Results Summary

Very bright markers that stains prostate basal epithelia

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Excellent specificity and brightness

The Bad

Other basal makers expressed more in basal epithelia leading to better separation

The Bottom Line

Great antibody

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