ODF2 Antibody

Cell Biology
Sloan-Kettering Institute
PHD Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Novus Biologicals

Product Name:

ODF2 Antibody (1A1)

Catalog Number:

Novus H00004957-M01

Browse Similar Products: ODF2 Immunofluorescence Antibodies

This ODF2 antibody stand the sub distal appendages of the centriole. Since the antibody is igg2a, it can be contained with a variety of other antibodies. Although it gives clear staining at centrioles, there is nonspecific labeling of nuclei.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

RPE1 cells, HeLa cells, Methanol Fixed

Primary Incubation

1:200 dilution of antibody in PBS with 3% BSA and 0.1% triton

Blocking Agent

PBS with 3% BSA and 0.1% triton

Secondary Incubation

Molecular probes anti-mouse Igg2a 594

Tertiary Incubation

DAPI

Detection

Fluorescent microscope

Results Summary

Antibody worked. Centrioles were stained but non-specific nuclear staining also present. Centriolar staining was not observed in ODF2 RNAi or CRISPR treated cells.

DOI or PMID #

27818179

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Stains subdistal appendages clearly

The Bad

Nonspecific foci in the nucleus

The Bottom Line

It is a good antibody of the igg2a isotope. Good for costainings.

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