Equine Immunophenotyping

June 16, 2017

Veterinary Diagnostic Laboratory
Kansas State University
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Company:

Novus Biologicals

Product Name:

CD44 Antibody (IM7) [FITC]

Catalog Number:

NBP1-41266F

Used in the phenotyping of equine whole blood cells. This antibody was used to gate out the leukocytic population in the cell sample.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Whole horse blood in EDTA

Primary Incubation

30 mins in dark at 4 degrees

Blocking Agent

NA

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

FACS calibur

Results Summary

This antibody was able to determine the leukcocyte population and helped us used that gate for looking at other subsets.

DOI or PMID #

NA

Additional Notes

NA

Related Categories

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Summary

The Good

Works well with horse blood

The Bad

None

The Bottom Line

Will re stock CD44 supply with this antibody.

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