Canine Immunophenotyping - Isotype control

Veterinary Diagnostic Laboratory
Kansas State University
RA

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Company:

Novus Biologicals

Product Name:

CD3 Antibody (CA17.2A12) [Allophycocyanin]

Catalog Number:

NBP2-49891APC

The aim of this experiment was to perform immunopenotyping of dog cells. We looked at dog lymphocytes and used this marker, CD3 to determine the T cell population in the lymphocytes. A high percentage of T cells was indicative of T cell lymphoma.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Whole dog blood in EDTA

Primary Incubation

30mins in dark at 4 degrees.

Blocking Agent

NA

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

FACS CAlibur

Results Summary

Was able to identify Tcell population with the use of this marker.

DOI or PMID #

NA

Additional Notes

NA

Related Categories

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Summary

The Good

Works well

The Bad

None

The Bottom Line

Will re purchase

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