Canine Immunophenotyping

Veterinary Diagnostic Laboratory
Kansas State University
RA

Overall

Quality of Results

Ease-of-Optimization

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Company:

Novus Biologicals

Product Name:

CD21 Antibody (Bu33) [Alexa Fluor® 647]

Catalog Number:

NB100-63574

The aim of this experiment was the immunophenotyping of dog blood cells, The CD21 maker used in this case helped us distinguish Bcells in the dog lymphocytes. This percentage of B cells would indicate presence of B cell lymphoma.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Whole dog blood in EDTA

Primary Incubation

30mins in dark

Blocking Agent

NA

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

BD fortessa

Results Summary

Shows true CD21+ staining in cells. Works well as a primary antibody.

DOI or PMID #

NA

Additional Notes

NA

Related Categories

Image Gallery

Summary

The Good

Works well

The Bad

None

The Bottom Line

Will buy again

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