Brilliant Violet 421™ anti-mouse CD3e Antibody for Flow Cytometry

May 31, 2017

Pathology
Stanford University
Postdoc

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

Brilliant Violet 421™ anti-mouse CD3ε Antibody

Catalog Number:

100335

I am interested in studying the immune system of the murine colonic lamina propria. Therefore, as a first step we had used a panel of antibodies to check the frequency of different types of immune cells.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse colon tissue sample

Primary Incubation

1:200 dilution; 20 min at 4 deg C

Blocking Agent

FcBlock for 5 min

Secondary Incubation

None

Tertiary Incubation

None

Detection

Flow Cytometry

Results Summary

CD3ε positive population is clearly distinguished with this antibody but the staining intensity is dim

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Clear stain

The Bad

Staining intensity is low

The Bottom Line

The antibody is mediocre!

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