Mouse B Cell IgG1 Expression

May 30, 2017

BlueskyReddit
Immunology
Northwestern University
Grad Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

BD Bioscience

Product Name:

PE Rat Anti-Mouse IgG1

Catalog Number:

550083

The aim of the research was the determine class switching to IgG1 in mouse splenic B cells after immunization with sheep red blood cells.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse spleen

Primary Incubation

1: 100, 4 degrees, 30 min

Blocking Agent

Fc block

Secondary Incubation

None

Tertiary Incubation

None

Detection

None

Results Summary

Good staining of IgG1 postive B cells

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Easy to use, clearly marks positive cells

The Bad

None

The Bottom Line

Great antibody for staining IgG1

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