Solid CD8 Percp Cy5.5 Antibody

June 01, 2017

GIVI
The Gladstone Institutes
Research Associate

Overall

Quality of Results

Ease-of-Optimization

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Company:

BD Biosciences

Product Name:

PerCP-Cy™5.5 Rat Anti-Mouse CD8a

Catalog Number:

561109

Our lab studies T-cell mediated immunity in the genital mucosa and so it is very important to have good and dependable antibodies for flow cytometry analysis.The purpose for this antibody is to isolate the populations of CD8 cells in mouse PBMCs.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse PBMCs

Primary Incubation

4 C for 30 minutes at 3:300 dilution

Blocking Agent

FC block (1:1000)

Secondary Incubation

None

Tertiary Incubation

None

Detection

Flow Cytometry

Results Summary

Our results indicated the existence of CD8 cells in our transgenic mice.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Antibody is great for detecting the existence of CD8 cells in otherwise KO animals

The Bad

None

The Bottom Line

This product has been very successful in interrogating mice lymphocytes and we'll definitely continue to use it in the future!

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