Great Antibody For B Cell Detection

May 16, 2017

Cell Biology
Albert Einstein College of Medicine
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

affymetrix eBioscience™ from Invitrogen Antibodies

Product Name:

CD45R (B220) Antibody, FITC (Monoclonal, RA3-6B2)

Catalog Number:

11-0452-82

The general aim of the research is to study hematopoiesis and acute myeloid leukemia. This antibody was used to detect B cells in murine peripheral blood.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Murine peripheral blood

Primary Incubation

1:100

Blocking Agent

Fc block is added to cells prior to staining with antibody

Secondary Incubation

None

Tertiary Incubation

None

Detection

Flow cytometry on a BD LSRII-yellow machine

Results Summary

This antibody gives a strong signal, allowing detection of a clear population of B cells.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Strong signal

The Bad

None

The Bottom Line

Highly recommended for murine B cell detection by flow cytometry

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