Great Antibody For Hematopoietic Stem Cell Sorting

May 15, 2017

BlueskyReddit
Cell Biology
Albert Einstein College of Medicine
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Biolegend

Product Name:

Brilliant Violet 421™ anti-mouse Ly-6A/E (Sca-1) Antibody

Catalog Number:

108127

The general aim of the research is to study hematopoiesis and acute myeloid leukemia. This antibody was used to isolate ckit+sca1+ cells from the bone marrow, a population that contains the hematopoietic stem and progenitor cells.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Murine bone marrow

Primary Incubation

1:100

Blocking Agent

None

Secondary Incubation

None

Tertiary Incubation

None

Detection

Flow cytometry on a BD LSRII-yellow machine

Results Summary

The antibody gives a strong signal and allows for easy isolation of the sca-1+ population

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Strong signal

The Bad

None

The Bottom Line

Recommended for detection and isolation of hematopoietic stem and progenitor cells

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