Biolegend
Brilliant Violet 421™ anti-mouse CD192 (CCR2) Antibody
150605
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My research aim is to investigate the response of the immune system after immune-therapy. This antibody is a good CCR2 marker, that has been tested on lymphocytes extracted from spleen, lymph nodes and glioma tumor-bearing mice with the aim to evaluate changes in the CCR2 expression on innate immune cells. Brilliant Violet 421™ anti-mouse CD192 (CCR2) has been chosen for the normally good quality/price of Biolegend antibody.
Flow Cytometry
Lymphocytes extracted from spleen, lymph nodes and brain tumor-bearing mice
30min
Fc Block
N/A
Fortessa
After cell extraction and blocking with Fc Block, wash your sample 2x with washing buffer, stain for 30min RT with your extracellular staining, wash 2x and then proceed with intracellular staining protocol for CCR2 (1:100).the data show CCR2+ macrophages of brain tumor-bearing mice in treated mice. Cells were gated for Live/CD45high/CD11d+/F4-80+
Clean positive population
Good antibody