eBioscience™ from Invitrogen Antibodies
46-0111-82
CD11a (LFA-1alpha) Monoclonal Antibody (M17/4), PerCP-eFluor 710
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Browse Similar Products: CD11a Flow Cytometry Antibodies
Our lab studies lymphocytes and their activity in both an active infection and the memory state. One of the markers we are interested in is CD11a and its expression on different lymphocyte populations. We chose this antibody with FITC because it is dim enough that this large population does not overwhelm other smaller populations.
Flow Cytometry
Mouse spleen cells
Cells were stained in 100uL FACS buffer with antibody at 1:200; for 1 hour on ice and in the dark. Cells were washed with FACS buffer and run in 100uL FACS buffer.
FACS buffer
None
BD Fortessa
We are able to use this body to clearly see a CD11a+ population of lymphocytes and measure how much CD11a+ is expressed by different cell populations in the spleen.
N/A
Low spillover
This is a good antibody for staining mouse spleen CD11a+ cells.