BD Biosciences
FITC anti-mouse CD11c
557400
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One of the focuses in our lab is studying lymphocytes and how they interact during an active influenza infection. We are able to use the CD11c marker to identify CD11c+ cells and differentiate them from T cells. We chose FITC because it is not too bright to overwhelm other cell populations.
Flow Cytometry
Mouse lung cells
Cells were stained in 100uL FACS buffer with antibody at 1:200; for 1 hour on ice and in the dark. Cells were washed with FACS buffer and run in 100uL FACS buffer.
FACS buffer
None
BD Fortessa
We were able to see a distinct population of CD11c+ cells with good separation.
N/A
Not much spill over
This is a good antibody for staining mouse CD11c+ cells from the lung.