IPO11 Immunohistochemistry Antibody

Cancer Centre
Cold Spring Harbor Laboratory
Lab Technician

Overall

Quality of Results

Ease-of-Optimization

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Company:

Abcam

Product Name:

IPO11

Catalog Number:

AP9661b

This antibody was selected because it can produce re-producable stains. We have been using it for years and work best compared to other brands.The goal is to create publishable data and this antibody is being used in future publications. IPO11 was used to ensure that our CRISPR knockout worked and was validated using the antibody.

Experimental Design and Results Summary

Applications

Immunohistochemistry

Sample

Mouse prostate

Primary Incubation

0.3% H2O2 in PBS

Blocking Agent

10% natural goat serum in PBS

Secondary Incubation

2nd biotin-conjugated antibody diluted (x400) in 1% BSA/PBS

Tertiary Incubation

VECTASTAIN ABC kit

Detection

DAB

Results Summary

IPO11 antibody worked well to detect deletion in our CRISPR model.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Best working antibody for IHC

The Bad

N/A

The Bottom Line

Use this antibody for best results of IPO11 detection.

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