LI-COR Biosciences
IRDye® 680LT Goat-anti-Rabbit Antibody
925-68021
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Our lab has generated mice that are infected with HIV at birth. We are interested at measuring HIV proteins such as p24 in different tissues to evaluate levels of infection and active replication. One of the detection methods is via western blot, and we use this antibody as secondary after the rabbit anti-p24 antibody.
Western Blot
Mouse spleen homogenate
Rabbit anti-p24
5% milk, 5% goat serum
Goat anti-rabbit
None
LI-COR Odyssey
Clean bands
N/A
Works very well, it is not easy to detect a lot of p24 in our system
I recommend it