eBioscience™ from Invitrogen Antibodies
Anti-Mouse V beta 5.1/5.2 TCR PerCP-eFluor® 710
46-5796-82
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Our lab studies the influenza virus and how T cells form immunological memory. One of our studies is the interaction of a specific TCR receptor with the virus upon infection. We use this antibody in combination with others to genotype mice for this specific TCR receptor. This fluorochrome works well with the other antibodies in our panel and is bright for these lower population of cells.
Flow Cytometry
Mouse blood
Cells were stained in 100uL FACS buffer with antibody at 1:100; for 30 minutes on ice and in the dark. Cells were washed with FACS buffer and run in 100uL FACS buffer.
FACS buffer
None
BD Fortessa
Using this antibody we are able to identify the TCR V beta 5 chain on T cells extracted from mouse blood.
N/A
Low spillover
Good antibody for TCR V beta chain on mouse T cells.