Good Antibody For Human Lung CD3 Cells

M&I
COLUMBIA UNIVERSITY
Lab Director / Manager

Overall

Quality of Results

Ease-of-Optimization

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Company:

BD Biosciences

Product Name:

anti-human CD3 BUV395

Catalog Number:

564001

Our lab studies various markers on lymphocytes extracted from human tissue samples. We need good markers to identify T cells specifically. We chose this antibody because it was very low spillover into other channels.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human lung lymphocytes

Primary Incubation

Cells were stained in 100uL FACS buffer with antibody at 1:50; for 20 minutes on ice and in the dark. Cells were washed with FACS buffer and run in 100uL FACS buffer.

Blocking Agent

FACS buffer

Secondary Incubation

None

Tertiary Incubation

None

Detection

LSR II

Results Summary

We were able to successfully mark and study CD3+ lymphocytes in different human lung tissues.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Low spillover

The Bad

None

The Bottom Line

This is a good antibody for large populations of CD3+ cells.

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