Anti C-terminal CD44 Antibody

May 04, 2017

The Cell and Molecular Biology
The Scripps Research Institute
Assistant Professor

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Quality of Results

Ease-of-Optimization

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Company:

Bioss Antibodies

Product Name:

CD44v10 Polyclonal Antibody

Catalog Number:

bs-2780R

The anti C-terminal CD44 antibody bs-2780R was used in parallel with the Boster antibody PB1021-2 to detect by western blotting the full-length CD44 and the cleaved membrane-retained CD44 fragment in human tumor cells.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Human cell lysates

Primary Incubation

Overnight, cold room

Blocking Agent

5% milk in PBS/0.05% Tween20

Secondary Incubation

goat anti-rabbit, 1 hr, room temperature

Tertiary Incubation

None

Detection

West pico

Results Summary

The bs-2780R failed to detect both the full-length CD44 and cleaved cell membrane-retained CD44 fragment in human tumor cells. In contrast, the Boster anti C-terminal CD44 antibody PB1021-2 reliably recognized CD44 in the same cell lysate samples.

DOI or PMID #

N/A

Additional Notes

The image demonstrates the full-length and cleaved CD44 detected by the Boster antibody and the lack of comparable CD44-related bands in the case of bs-2780R antibody.

Related Categories

Image Gallery

Summary

The Good

None

The Bad

The antibody failed to reliably detect by western blotting CD44 expressed on the surface of human tumor cells.

The Bottom Line

We would not recommend this antibody for reliable detection by western blotting of cell surface CD44 in human tumor cells.

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