Aviva Systems Biology
CD44 antibody C-terminal region
ARP61023_P050
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The ARP61023_P050 antibody was compared in western blot assay with the C-terminal CD44 antibody from Boster proven to detect CD44 localized on the cell membrane of human and murine tumor cells.
Western Blot
Human and murine cell lysates
Overnight, cold room
5% milk in PBS/0.05% Tween20
Goat anti-rabbit, 1 hr, room temperature
None
West pico
The ARP61023_PO50 failed to detect both full-length CD44 and cleaved cell membrane-retained CD44 fragment in human tumor cells. In contrast, the Boster anti C-terminal CD44 antibody PB1021-2 reliably recognized CD44 in the same cell lysate samples.
N/A
The image demonstrates the full-length and cleaved CD44 detected by the Boster antibody and the lack of comparable CD44-related bands in the case of Aviva antibody.
Nothing noted
This antibody did not work in our hands
We would not recommend this antibody for reliable detection of CD44 by western blotting in cell lysates.