PBL Assay Science
Human IFN Alpha 2
11105-1
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I use this hIFNa in both the development and quality control of our reporter cell lines. A dose response curve is generated for each new lot of cells produced to insure that the cells are performing consistently from lot to lot. It is critical that that the ligands used to stimulate the cells can produce highly consistent results to insure that each new lot is properly evaluated. I choose to use the PBL brand IFNa because it has always given us excellent, reliable results and the price is reasonable.
Quality control
HEK293 reporter cell line
I perform a serial dilution of the hIFNa and add the dilutions to a 96-well plate. 50,000 cells of the appropriate cell line are then added to each well. The media added to the wells is designed for the detection of NF-κB induced SEAP expression. After a 16-24 hr incubation the optical density (OD) is read at 650 nm.
It is important to aliquot the IFN upon receiving the fresh vial and store at -80C if you do not plan to use the full vial in a single experiment. This will allow you to have consistent results between assays that use aliquots from the same source vial.
PBL human IFNa2 gives me a high level of reproducibility between assays.
N/A
None
Consistent results, high quality product.
None.
A wonderful standard for cell-based assays when IFNa is required.