Anti-TCRb Identifies T Cells In The Lymph Node Very Well

May 02, 2017

Pathology
Stanford
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Biolegend

Product Name:

APC anti-mouse TCR β chain Antibody

Catalog Number:

109212

We wanted to examine the lymph nodes in a diseased vs healthy state, and T cells were one cell of interest, leading us to use the antibody.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Single-cell suspension of mouse lymph node cells

Primary Incubation

1:100 in staining buffer for 25 minutes at 4 degrees

Blocking Agent

FcR Block

Secondary Incubation

None

Tertiary Incubation

None

Detection

BD LSR II flow cytometer

Results Summary

The T cells stained very well with the antibody

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Stains T cells very well

The Bad

None to mention

The Bottom Line

Great antibody for pulling out T cells clearly

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