Thermo Fisher Scientific
Cell Stimulation Cocktail (plus protein transport inhibitors) (500X)
00-4975-03
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This was used to restimulate total splenocytes in vitro in order to measure cytokines.
Flow cytometry
Mouse splenocytes
This product was used at 500x and cells were incubated overnight or 4 hours at 37C prior to washing and staining.
Can be used at 2 fold lower dose with same results
This cocktail is too strong of an activator, leading to a ton of cell death.
N/A
Easy to use
Increased cell death
Try another stimulation